The 1995 Eastern Illinois Panthers football team represented Eastern Illinois University as a member of the Gateway Football Conference during the 1995 NCAA Division I-AA football season. Led by ninth-year head coach Bob Spoo, the Panthers compiled and overall record of 10–2 with a mark of 5–1 in conference play, winning the Gateway title. Eastern Illinois was invited to the NCAA Division I-AA Football Championship playoffs, where they lost to Stephen F. Austin in the first round.
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